BMRB Entry 26692

Title:
Solid State NMR sequential assignment of Amyloid b(1-42) fibrils
Deposition date:
2015-10-23
Original release date:
2016-05-24
Authors:
Ravotti, Francesco; Waelti, Marielle; Guentert, Peter; Meier, Beat; Riek, Roland; Boeckmann, Anja
Citation:

Citation: Ravotti, Francesco; Waelti, Marielle; Guentert, Peter; Meier, Beat; Riek, Roland; Boeckmann, Anja. "Solid-State NMR sequential assignment of Amyloid-b (1-42) fibrils"  Biomol. NMR Assign. 10, 269-276 (2016).
PubMed: 27165577

Assembly members:

Assembly members:
Amyloid_Beta, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Amyloid_Beta: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV IA

Data sets:
Data typeCount
13C chemical shifts169
15N chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Amyloid Beta fibril1

Entities:

Entity 1, Amyloid Beta fibril 42 residues - Formula weight is not available

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL
5   ILEALA

Samples:

sample_1: Amyloid Beta, [U-13C], 20 mg

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1
NCACBsample_1isotropicsample_conditions_1
CCCsample_1isotropicsample_conditions_1
NCAsample_1isotropicsample_conditions_1
NCOsample_1isotropicsample_conditions_1
DARRsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin, CCPN - chemical shift assignment, data analysis, processing

NMR spectrometers:

  • Bruker Avance 850 MHz